<div dir="ltr"><div style>Hi Ensembl devs!</div><div style><br></div><div style>I've got some troubles with Ensembl Genomes BLAST (for fungi, protists, ...)</div><div style><br></div><div style>When I run the BLAST tool it crashes after the alignment step, when tries to parse the <i>blastall</i> results.</div>
<div style>I've repeated manually the <i>blastall</i> command (below these lines) and it works correctly, returning the alignments, so the error should be after this step...</div><div style><br></div><div style><span style="font-family:'courier new',monospace;font-size:x-small">export PATH=/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin:/opt/X11/bin:/Applications/Server.app/Contents/ServerRoot/usr/bin:/Applications/Server.app/Contents/ServerRoot/usr/sbin:/opt/local/bin/:/opt/local/sbin/:/usr/local/ncbi/blast/bin/:/usr/local/biotools/blast/blast/bin/:/local/bin:/local/bin/wu_blast:/local/bin:/local/bin/wu_blast; export BLASTMAT=/local/bin/wu_blast/matrix; export BLASTFILTER=/local/bin/wu_blast/filter; export BLASTDB=/usr/local/blastdb/ensembl_protists; cat /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fasta | blastall -d Plasmodium_falciparum.ASM276v1.17.dna.toplevel.fa -p blastn -b 250 -e 10</span><br>
</div><div style><span style="font-family:'courier new',monospace;font-size:x-small"><br></span></div><div style><span style="font-family:'courier new',monospace;font-size:x-small"><br></span></div><div style>
Could be a configuration error? </div><div style>My MULTI.ini file only contains the following settings, maybe something is misconfigured (maybe Ensembl tryes to parse the output of another BLAST method instead ncbi blastn output...).</div>
<div style><font face="courier new, monospace" size="1"><br></font></div><div style><font face="courier new, monospace" size="1"> [BLAST_DATASOURCES]</font></div><div><font face="courier new, monospace" size="1">; Registers blast datasources. Key values are used as labels</font></div>
<div><font face="courier new, monospace" size="1">; Keys should be registered against methods in species.ini files</font></div><div><font face="courier new, monospace" size="1">DEFAULT = LATESTGP</font></div><div>
<font face="courier new, monospace" size="1">CDNA_ALL = cDNAs</font></div><div><font face="courier new, monospace" size="1">CDNA_ABINITIO = Ab-initio cDNAs (Genscan/SNAP)</font></div><div><font face="courier new, monospace" size="1">PEP_ALL = Peptides</font></div>
<div><font face="courier new, monospace" size="1">PEP_ABINITIO = Ab-initio Peptides (Genscan/SNAP)</font></div><div><font face="courier new, monospace" size="1">RNA_NC = Ensembl Non-coding RNA genes</font></div>
<div><font face="courier new, monospace" size="1">LATESTGP = Genomic sequence</font></div><div><font face="courier new, monospace" size="1">LATESTGP_MASKED = Genomic sequence (masked)</font></div><div><font face="courier new, monospace" size="1"><br>
</font></div><div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">[ENSEMBL_BLAST_METHODS]</font></div><div><font face="courier new, monospace" size="1">BLASTN = [blast dna dna ncbiblastn] ; alternatives: ncbiblastn, ensembl_wublastn, sge_wublastn</font></div>
<div><font face="courier new, monospace" size="1">BLASTX = [blast dna peptide ncbiblastx] ; alternatives: ncbiblastx, ensembl_wublastx, sge_wublastx</font></div><div><font face="courier new, monospace" size="1">BLASTP = [blast peptide peptide ncbiblastp] ; alternatives: ncbiblastp, ensembl_wublastp, sge_wublastp</font></div>
<div><font face="courier new, monospace" size="1">TBLASTN = [blast peptide dna ncbitblastn] ; alternatives: ncbitblastn, ensembl_wutblastn, sge_wutblastn</font></div><div><font face="courier new, monospace" size="1">TBLASTX = [blast dna dna ncbitblastx] ; alternatives: ncbitblastx, ensembl_wutblastx, sge_wutblastx</font></div>
<div><font face="courier new, monospace" size="1">BLAT = [blat dna dna blat_gfclient]</font></div><div><br></div><div><font face="courier new, monospace" size="1">[databases]</font></div><div><font face="courier new, monospace" size="1">DATABASE_BLAST = ensembl_blast</font></div>
<div><font face="courier new, monospace" size="1">[DATABASE_BLAST]</font></div><div><font face="courier new, monospace" size="1">HOST = localhost </font></div><div><font face="courier new, monospace" size="1">PORT = 3306</font></div>
<div><font face="courier new, monospace" size="1">USER = ***********</font></div><div><font face="courier new, monospace" size="1">PASS = ***********</font></div><div><br></div><div style><br></div><div style><span style="font-family:arial,sans-serif;font-size:12.727272033691406px">BTW,</span><span style="font-family:arial,sans-serif;font-size:12.727272033691406px"> i</span>n spite of this configuration, in the BLAST page the combobox for the <i>dna database</i> (LATEST GP, ...) or <i>protein database </i>selection are empty (I'll open another thread for this issue).</div>
<div style><br></div><div style>Below the error log: </div><div style><br></div><div style><font face="courier new, monospace" size="1"><br></font></div><div style><div><font face="courier new, monospace" size="1">db: Plasmodium_falciparum_LATESTGP </font></div>
<div><font face="courier new, monospace" size="1"> dbprefix: LATESTGP </font></div><div><font face="courier new, monospace" size="1"> db_key: </font></div><div><font face="courier new, monospace" size="1"> sp: Plasmodium_falciparum </font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">BLA_LjKT7L2A1 at /usr/local/ensembl_protists/perl/Multi/blastview line 272.</font></div>
<div><font face="courier new, monospace" size="1">STORING BLAST: BLA_LjKT7L2A1 [PID=3860 TIME=301800054] at /usr/local/ensembl_protists/perl/Multi/blastview line 274.</font></div><div><font face="courier new, monospace" size="1">/usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y at /usr/local/ensembl_protists/perl/Multi/blastview line 279.</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">RUNNING Bio::Tools::Run::Search::ncbiblastn=HASH(0x7fb9346188e8) at /usr/local/ensembl_protists/perl/Multi/blastview line 280.</font></div>
<div><font face="courier new, monospace" size="1">==> export PATH=/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin:/opt/X11/bin:/Applications/Server.app/Contents/ServerRoot/usr/bin:/Applications/Server.app/Contents/ServerRoot/usr/sbin:/opt/local/bin/:/opt/local/sbin/:/usr/local/ncbi/blast/bin/:/usr/local/biotools/blast/blast/bin/:/local/bin:/local/bin/wu_blast:/local/bin:/local/bin/wu_blast; export BLASTMAT=/local/bin/wu_blast/matrix; export BLASTFILTER=/local/bin/wu_blast/filter; export BLASTDB=/usr/local/blastdb/ensembl_protists; cat /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fasta | blastall -d Plasmodium_falciparum.ASM276v1.17.dna.toplevel.fa -p blastn -b 250 -e 10 > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ 2>&1 ; mv /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out at /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search/NCBIBlast.pm line 223.</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">--------------------- WARNING ---------------------</font></div><div><font face="courier new, monospace" size="1">MSG: Command export PATH=/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin:/opt/X11/bin:/Applications/Server.app/Contents/ServerRoot/usr/bin:/Applications/Server.app/Contents/ServerRoot/usr/sbin:/opt/local/bin/:/opt/local/sbin/:/usr/local/ncbi/blast/bin/:/usr/local/biotools/blast/blast/bin/:/local/bin:/local/bin/wu_blast:/local/bin:/local/bin/wu_blast; export BLASTMAT=/local/bin/wu_blast/matrix; export BLASTFILTER=/local/bin/wu_blast/filter; export BLASTDB=/usr/local/blastdb/ensembl_protists; cat /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fasta | blastall -d Plasmodium_falciparum.ASM276v1.17.dna.toplevel.fa -p blastn -b 250 -e 10 > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ 2>&1 ; mv /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">------------- EXCEPTION: Bio::Root::Exception -------------</font></div><div><font face="courier new, monospace" size="1">MSG: Parsing failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">-------------------- EXCEPTION --------------------</font></div><div><font face="courier new, monospace" size="1">MSG: seq_region_name argument is required</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::EnsEMBL::DBSQL::SliceAdaptor::fetch_by_region /usr/local/ensembl_protists/ensembl/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm:201</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Search::HSP::EnsemblHSP::_map /usr/local/ensembl_protists/eg-plugins/common/modules/Bio/Search/HSP/EnsemblHSP.pm:435</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::Search::Result::EnsemblResult::map_to_genome /usr/local/ensembl_protists/modules/Bio/Search/Result/EnsemblResult.pm:271</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::parse /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1082</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) (eval 725):1</font></div><div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/SpeciesHandler.pm:123</font></div>
<div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div>
<div><font face="courier new, monospace" size="1">STACK toplevel /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">Date (localtime) = Mon Mar 4 13:27:35 2013</font></div>
<div><font face="courier new, monospace" size="1">Ensembl API version = 70</font></div><div><font face="courier new, monospace" size="1">---------------------------------------------------</font></div><div><font face="courier new, monospace" size="1"><br>
</font></div><div><font face="courier new, monospace" size="1">STACK: Error::throw</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Root::Root::throw /Library/Perl/5.12/Bio/Root/Root.pm:472</font></div>
<div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1019</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div>
<div><font face="courier new, monospace" size="1">STACK: /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">-----------------------------------------------------------</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">---------------------------------------------------</font></div><div><font face="courier new, monospace" size="1"><br>
</font></div><div><font face="courier new, monospace" size="1">RUN Bio::Tools::Run::Search::ncbiblastn=HASH(0x7fb9346188e8) at /usr/local/ensembl_protists/perl/Multi/blastview line 282.</font></div><div><font face="courier new, monospace" size="1">HERE.... FAILED at /usr/local/ensembl_protists/perl/Multi/blastview line 287.</font></div>
<div><font face="courier new, monospace" size="1">Bio::Tools::Run::Search::ncbiblastn at /usr/local/ensembl_protists/perl/Multi/blastview line 288.</font></div><div><font face="courier new, monospace" size="1">STORED.... at /usr/local/ensembl_protists/perl/Multi/blastview line 290.</font></div>
<div><font face="courier new, monospace" size="1">*** BLAST ERROR: /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y ***</font></div><div><font face="courier new, monospace" size="1">Blast job resulted in /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fail file:</font></div>
<div><font face="courier new, monospace" size="1">Command export PATH=/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin:/opt/X11/bin:/Applications/Server.app/Contents/ServerRoot/usr/bin:/Applications/Server.app/Contents/ServerRoot/usr/sbin:/opt/local/bin/:/opt/local/sbin/:/usr/local/ncbi/blast/bin/:/usr/local/biotools/blast/blast/bin/:/local/bin:/local/bin/wu_blast:/local/bin:/local/bin/wu_blast; export BLASTMAT=/local/bin/wu_blast/matrix; export BLASTFILTER=/local/bin/wu_blast/filter; export BLASTDB=/usr/local/blastdb/ensembl_protists; cat /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fasta | blastall -d Plasmodium_falciparum.ASM276v1.17.dna.toplevel.fa -p blastn -b 250 -e 10 > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ 2>&1 ; mv /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">------------- EXCEPTION: Bio::Root::Exception -------------</font></div><div><font face="courier new, monospace" size="1">MSG: Parsing failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">-------------------- EXCEPTION --------------------</font></div><div><font face="courier new, monospace" size="1">MSG: seq_region_name argument is required</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::EnsEMBL::DBSQL::SliceAdaptor::fetch_by_region /usr/local/ensembl_protists/ensembl/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm:201</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Search::HSP::EnsemblHSP::_map /usr/local/ensembl_protists/eg-plugins/common/modules/Bio/Search/HSP/EnsemblHSP.pm:435</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::Search::Result::EnsemblResult::map_to_genome /usr/local/ensembl_protists/modules/Bio/Search/Result/EnsemblResult.pm:271</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::parse /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1082</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) (eval 725):1</font></div><div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/SpeciesHandler.pm:123</font></div>
<div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div>
<div><font face="courier new, monospace" size="1">STACK toplevel /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">Date (localtime) = Mon Mar 4 13:27:35 2013</font></div>
<div><font face="courier new, monospace" size="1">Ensembl API version = 70</font></div><div><font face="courier new, monospace" size="1">---------------------------------------------------</font></div><div><font face="courier new, monospace" size="1"><br>
</font></div><div><font face="courier new, monospace" size="1">STACK: Error::throw</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Root::Root::throw /Library/Perl/5.12/Bio/Root/Root.pm:472</font></div>
<div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1019</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div>
<div><font face="courier new, monospace" size="1">STACK: /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">-----------------------------------------------------------</font></div>
<div><font face="courier new, monospace" size="1">*** BLAST ERROR: /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y ***</font></div><div><font face="courier new, monospace" size="1">Blast job resulted in /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fail file:</font></div>
<div><font face="courier new, monospace" size="1">Command export PATH=/usr/bin:/bin:/usr/sbin:/sbin:/usr/local/bin:/opt/X11/bin:/Applications/Server.app/Contents/ServerRoot/usr/bin:/Applications/Server.app/Contents/ServerRoot/usr/sbin:/opt/local/bin/:/opt/local/sbin/:/usr/local/ncbi/blast/bin/:/usr/local/biotools/blast/blast/bin/:/local/bin:/local/bin/wu_blast:/local/bin:/local/bin/wu_blast; export BLASTMAT=/local/bin/wu_blast/matrix; export BLASTFILTER=/local/bin/wu_blast/filter; export BLASTDB=/usr/local/blastdb/ensembl_protists; cat /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.fasta | blastall -d Plasmodium_falciparum.ASM276v1.17.dna.toplevel.fa -p blastn -b 250 -e 10 > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out > /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ 2>&1 ; mv /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out_ /usr/local/ensembl_protists/blastqueue/BLA_Lj/KT7L2A1/sZC1hF1Y.out failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">------------- EXCEPTION: Bio::Root::Exception -------------</font></div><div><font face="courier new, monospace" size="1">MSG: Parsing failed</font></div>
<div><font face="courier new, monospace" size="1"><br></font></div><div><font face="courier new, monospace" size="1">-------------------- EXCEPTION --------------------</font></div><div><font face="courier new, monospace" size="1">MSG: seq_region_name argument is required</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::EnsEMBL::DBSQL::SliceAdaptor::fetch_by_region /usr/local/ensembl_protists/ensembl/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm:201</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Search::HSP::EnsemblHSP::_map /usr/local/ensembl_protists/eg-plugins/common/modules/Bio/Search/HSP/EnsemblHSP.pm:435</font></div>
<div><font face="courier new, monospace" size="1">STACK Bio::Search::Result::EnsemblResult::map_to_genome /usr/local/ensembl_protists/modules/Bio/Search/Result/EnsemblResult.pm:271</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::parse /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1082</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1005</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div><div><font face="courier new, monospace" size="1">STACK Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div>
<div><font face="courier new, monospace" size="1">STACK (eval) (eval 725):1</font></div><div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/SpeciesHandler.pm:123</font></div>
<div><font face="courier new, monospace" size="1">STACK EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div><div><font face="courier new, monospace" size="1">STACK (eval) /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div>
<div><font face="courier new, monospace" size="1">STACK toplevel /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">Date (localtime) = Mon Mar 4 13:27:35 2013</font></div>
<div><font face="courier new, monospace" size="1">Ensembl API version = 70</font></div><div><font face="courier new, monospace" size="1">---------------------------------------------------</font></div><div><font face="courier new, monospace" size="1"><br>
</font></div><div><font face="courier new, monospace" size="1">STACK: Error::throw</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Root::Root::throw /Library/Perl/5.12/Bio/Root/Root.pm:472</font></div>
<div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::dispatch /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:1019</font></div><div><font face="courier new, monospace" size="1">STACK: Bio::Tools::Run::Search::run /usr/local/ensembl_protists/modules/Bio/Tools/Run/Search.pm:935</font></div>
<div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::SpeciesHandler::handler_species /usr/local/ensembl_protists/perl/Multi/blastview:281</font></div><div><font face="courier new, monospace" size="1">STACK: EnsEMBL::Web::Apache::Handlers::handler /usr/local/ensembl_protists/modules/EnsEMBL/Web/Apache/Handlers.pm:369</font></div>
<div><font face="courier new, monospace" size="1">STACK: /usr/local/ensembl_protists/perl/Multi/blastview:0</font></div><div><font face="courier new, monospace" size="1">-----------------------------------------------------------</font></div>
<div><br></div></div><div style>Thank you again,</div><div style><br></div><div style>Best regards</div><br clear="all"><div><font style="color:rgb(102,102,102)"><span style="font-family:tahoma,sans-serif">Rafa Hernández de Diego </span></font><br>
<div><span style="color:rgb(153,153,153)"><br></span></div><div><font color="#999999"><span style="font-family:arial,sans-serif;font-size:12.727272033691406px">SLU-Global Bioinformatics Center</span><br style="font-family:arial,sans-serif;font-size:12.727272033691406px">
</font><span style="font-family:arial,sans-serif;font-size:12.727272033691406px;color:rgb(153,153,153)">Swedish University for Agricultural Sciences (SLU)</span><font color="#999999"><br><span style="font-family:arial,sans-serif;font-size:12.727272033691406px">Gerda Nilssons väg 2, </span><br style="font-family:arial,sans-serif;font-size:12.727272033691406px">
<span style="font-family:arial,sans-serif;font-size:12.727272033691406px"> Box 7023, 750 07 Uppsala, Sweden.</span></font><br></div></div>
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