<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div><span><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;">Hi,</div><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;">I can run the code some days ago . But cant run now. </div><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;"><br></div><div style="background-color: transparent; font-family: arial, helvetica, sans-serif; font-size: 13px;"><span style="font-weight: bold; text-decoration: underline;">Problem 1:</span> Output is ok</div><div style="background-color: transparent; font-family: arial, helvetica, sans-serif; font-size: 13px;">ensembl = useDataset("hsapiens_gene_ensembl",mart=ensembl)</div><div style="font-size: 12pt; background-color: transparent;"><font size="2">utr5 = getSequence(chromosome=3, start=185514033,
end=185535839, </font><span style="background-color: transparent;"><font size="2">type="entrezgene",seqType="5utr", mart=ensembl)</font></span><font size="2" style="background-color: transparent;"> </font></div><div style="background-color: transparent; font-family: arial, helvetica, sans-serif; font-size: 13px;"><font size="2" style="background-color: transparent;"><span style="text-decoration: underline;">Output :</span> </font></div><div style="font-size: 12pt; background-color: transparent;"><font size="2" style="background-color: transparent;"><div style="text-align: right; background-color: transparent;"> 5utr
entrezgene</div><div style="text-align: right; background-color: transparent;"> Sequence unavailable 10644</div><div style="text-align: right; background-color: transparent;"> GGAGCGCCGGGTACCGGGCCGGGGGAGCCGCGGGCTCTCGGGGAAGAGACGG 10644</div><div style="text-align: right; background-color: transparent;">3 GGGGGGCGGAGGAGGAGGAGAGACGAGGGCAGCGGAGGAGGCGAGGAGCGCCGGGTACCGGGCCGGGGGAGCCGCGGGCTCTCGGGGAAGAGACGG 10644</div><div style="text-align: right; background-color:
transparent;"> CGGAGGAGGCGAGGAGCGCCGGGTACCGGGCCGGGGGAGCCGCGGGCTCTCGGGGAAGAGACGG 10644</div><div style="text-align: right; background-color: transparent;"> No UTR is annotated for this transcript 10644</div></font></div><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;"></div><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;"> </div><div style="font-family: arial, helvetica, sans-serif; font-size: 10pt;"><span style="font-weight: bold; text-decoration: underline;">Problem 2:Problem is here</span><br></div><div style="background-color:
transparent;"><span><div style="font-size: 13px; background-color: transparent;">protein = getSequence(id=c(100, 5728),type="entrezgene",<span style="background-color: transparent;">seqType="peptide", mart=ensembl)</span></div><div style="font-size: 13px; background-color: transparent; font-family: arial, helvetica, sans-serif;"><span style="background-color: transparent;"><br></span></div><div style="background-color: transparent;"><span style="background-color: transparent; font-size: 13px;"><div style="background-color: transparent;">Error in getBM(c(seqType, type), filters = type, values = id, mart = mart, : </div><div style="background-color: transparent;"> Query ERROR: caught BioMart::Exception::Database: Error during query execution: Can't create/write to file '/mnt/ephemeral0/mysqltmp/#sql_40a_0.MYI' (Errcode: 2)</div><div style="background-color: transparent;"><br></div><div style="background-color: transparent; font-family:
arial, helvetica, sans-serif;">I need help please.</div></span></div></span></div></span></div><div></div><div> </div><div><span style="font-family: 'times new roman'; font-weight: bold;"><i>/.......Tanvir Ahamed</i></span><br></div></div></body></html>