<div dir="ltr">Hi,<div><br></div><div>While transmembrane regions in Ensembl protein summary are based on TMHMM:<br></div><div><br></div><div><a href="http://www.ensembl.org/Help/View?id=177">http://www.ensembl.org/Help/View?id=177</a></div>
<div><br clear="all"><div>It is interesting to find that for some proteins with established number of transmemrbane helices such as GPCRs e.g. , Ensembl shows 6 predicted TMs for the merged ensembl-havana transcript protein ENSP00000355316. The corresponding Uniprot ID (Q14832) shows the correct number of 7 TM helices:</div>
<div><div><br></div><div>TMH1</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>577 – 599<span class="" style="white-space:pre">       </span>23<span class="" style="white-space:pre">        </span>Helical; Name=1; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>577<span class="" style="white-space:pre">       </span>599</div><div>TMH2</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>614 – 634<span class="" style="white-space:pre">       </span>21<span class="" style="white-space:pre">        </span>Helical; Name=2; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>612<span class="" style="white-space:pre">       </span>634</div><div>TMH3</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>646 – 664<span class="" style="white-space:pre">       </span>19<span class="" style="white-space:pre">        </span>Helical; Name=3; Potential</div>
<div>TMH4</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">    </span>689 – 709<span class="" style="white-space:pre">       </span>21<span class="" style="white-space:pre">        </span>Helical; Name=4; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>687<span class="" style="white-space:pre">       </span>709</div><div>TMH5</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>735 – 756<span class="" style="white-space:pre">       </span>22<span class="" style="white-space:pre">        </span>Helical; Name=5; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>737<span class="" style="white-space:pre">       </span>756</div><div>TMH6</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>770 – 792<span class="" style="white-space:pre">       </span>23<span class="" style="white-space:pre">        </span>Helical; Name=6; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>771<span class="" style="white-space:pre">       </span>793</div><div>TMH7</div><div>Uniprot Transmembrane<span class="" style="white-space:pre">        </span>803 – 828<span class="" style="white-space:pre">       </span>26<span class="" style="white-space:pre">        </span>Helical; Name=7; Potential</div>
<div>Ensembl Transmembrane<span class="" style="white-space:pre">       </span>806<span class="" style="white-space:pre">       </span>828</div><div><br></div><div><a href="http://www.uniprot.org/uniprot/Q14832">http://www.uniprot.org/uniprot/Q14832</a></div>
</div><div><br></div><div>Would be useful if protein features from sources such as uniprot can be checked against/incorporated into Ensembl.</div><div><br></div><div>Best regards</div><div><br></div>-- <br><div dir="ltr">
G.</div>
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