<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Dear Guillermo,<div><br></div><div><span style="line-height: 16.0029983520508px;">I am afraid we have renamed the "external_gene_id" attribute internal name to "external_gene_name" in release 76. Renaming the attribute in your script should resolve your problem.</span></div><div><span style="line-height: 16px;">We rarely rename attribute internal name but I am afraid we don't declare these at the moment since they don't impact the BioMart visual interface. If an important filter/attribute is remove from the interface or renamed on the interface then we always declare it.</span></div><div><span style="line-height: 16px;"><br></span></div><div><span style="line-height: 16px;">Apologies for any inconvenience cause,</span></div><div><span style="line-height: 16px;">Regards,</span></div><div><span style="line-height: 16px;">Thomas<br></span><div><div>On 24 Sep 2014, at 12:13, Guillermo Marco Puche <<a href="mailto:guillermo.marco@sistemasgenomicos.com">guillermo.marco@sistemasgenomicos.com</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
  
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    Dear Thomas,<br>
    <br>
    I'm experiencing an error when querying to BioMart Ensembl 76
    registry for both clean/cached.<br>
    Using older Mart Registry to ensembl 75 registry  archive works for
    both clean/cached.<br>
    <br>
        my $action='clean';<br>
    <blockquote>
      <blockquote>    my $initializer =
        BioMart::Initializer->new('registryFile'=>$confFile,
        'action'=>$action);<br>
            my $registry = $initializer->getRegistry;<br>
        <br>
            my $query =
BioMart::Query->new('registry'=>$registry,'virtualSchemaName'=>'default');<br>
        <br>
                $query->setDataset("hsapiens_gene_ensembl");<br>
                if ( length($gene_name)==15 &&
        substr($gene_name,0,4) eq "ENSG" ) {
        $query->addFilter("ensembl_gene_id", @{$gene}); }<br>
                else { $query->addFilter("hgnc_symbol", @{$gene}); }<br>
                if ($query_type eq "gene_info"){<br>
                    #$query->addFilter("biotype",
        ["protein_coding"]);<br>
                    $query->addAttribute("ensembl_gene_id");<br>
                    $query->addAttribute("ensembl_transcript_id");<br>
                    $query->addAttribute("chromosome_name");<br>
                    <b>$query->addAttribute("external_gene_id");</b><br>
                }<br>
      </blockquote>
    </blockquote>
    Was this removed in Ensembl 76? Is there any documentation about
    BioMart available queries per Ensembl release?<br>
    <br>
    Thank you and sorry for spam, I never had this issues when changing
    Ensembl previously.<br>
    <br>
    Best regards,<br>
    Guillermo.<br>
    <br>
    <br>
    <div class="moz-cite-prefix">On 23/09/14 15:10, Guillermo Marco
      Puche wrote:<br>
    </div>
    <blockquote cite="mid:5421714A.7050200@sistemasgenomicos.com" type="cite">
      <meta content="text/html; charset=windows-1252" http-equiv="Content-Type">
      Dear Thomas,<br>
      <br>
      Ok I didn't understand correctly, now I do.<br>
      As you say biomart website registry is still showing ensembl 75.<br>
      <br>
      Thank you.<br>
      <br>
      Best regards,<br>
      Guillermo.<br>
      <br>
      <div class="moz-cite-prefix">On 23/09/14 14:01, Thomas Maurel
        wrote:<br>
      </div>
      <blockquote cite="mid:F1A87ED1-E57D-4289-8CCD-2965E6FAAC28@ebi.ac.uk" type="cite">
        <meta http-equiv="Content-Type" content="text/html;
          charset=windows-1252">
        Dear Guillermo,
        <div><br>
        </div>
        <div>The <a moz-do-not-send="true" href="http://biomart.org/">biomart.org</a>
          website seems to be very slow at the moment and I am afraid
          the website is still displaying our release 75 marts on hg19
          (GRCh37). According to the <a moz-do-not-send="true" href="http://biomart.org/">biomart.org</a> mart registry
          page: <a moz-do-not-send="true" href="http://www.biomart.org/biomart/martservice?type=registry">http://www.biomart.org/biomart/martservice?type=registry</a>, port

          '80' is still valid.</div>
        <div>If you want to use hg38 (GRCh38), the best way would be to
          point your mart XML config to the <a moz-do-not-send="true" href="http://ensembl.org/">ensembl.org</a> website in order
          to access our release 76 mart databases on hg38 (GRCh38).</div>
        <div>You can follow the previous instruction but just change the
          mart registry URL page to get the mart release 76 registry
          informations:</div>
        <div><br>
        </div>
        <div>
          <blockquote type="cite">
            <div text="#000066" bgcolor="#FFFFFF">
              <blockquote cite="mid:4B2EFDEF-33F9-4F59-8B37-30795F2DA7CA@ebi.ac.uk" type="cite">2) A BioMart perl script
                <div>a) You first need to edit your configuration file
                  in "biomart-perl/conf/martURLLocation.xml" and paste
                  the content of the mart registry page for the </div>
              </blockquote>
            </div>
          </blockquote>
          <div text="#000066" bgcolor="#FFFFFF">
            <div>Ensembl website on release 76 (hg38): <a moz-do-not-send="true" href="http://www.ensembl.org/biomart/martservice?type=registry">http://www.ensembl.org/biomart/martservice?type=registry</a></div>
          </div>
          <blockquote type="cite">
            <div text="#000066" bgcolor="#FFFFFF">
              <blockquote cite="mid:4B2EFDEF-33F9-4F59-8B37-30795F2DA7CA@ebi.ac.uk" type="cite">
                <div>b) Then edit your script and make sure that "my
                  $confFile" variable is looking at
                  the martURLLocation.xml configuration file in
                  biomart-perl/conf</div>
                <div>c) Finally, make sure to update the following line
                  in your script:</div>
                <div><br>
                </div>
                <div>my $action='cached';</div>
                <div>
                  <div><br>
                  </div>
                  <div>with:</div>
                  <div><br>
                  </div>
                  <div>my $action='clean';</div>
                  <div>
                    <div><br>
                    </div>
                    <div>The first run of your script on Ensembl 75
                      might be a bit slow as BioMart will cache some
                      data from the BioMart website.</div>
                    <div>Once you have run your script with the action
                      variable set to "clean", you can set the variable
                      to "cached" again.</div>
                  </div>
                </div>
              </blockquote>
            </div>
          </blockquote>
        </div>
        <div><br>
        </div>
        <div>I am afraid $action=clean is quite resource consuming but
          you will only need to run your script with this setting when
          you change the registry information.</div>
        <div><br>
        </div>
        <div>Hope this helps,</div>
        <div>Best regards,</div>
        <div>Thomas</div>
        <div>
          <div>
            <div>On 23 Sep 2014, at 12:43, Guillermo Marco Puche <<a moz-do-not-send="true" href="mailto:guillermo.marco@sistemasgenomicos.com">guillermo.marco@sistemasgenomicos.com</a>>

              wrote:</div>
            <br class="Apple-interchange-newline">
            <blockquote type="cite">
              <meta content="text/html; charset=windows-1252" http-equiv="Content-Type">
              <div text="#000066" bgcolor="#FFFFFF"> Dear Thomas,<br>
                <br>
                My BioMart perl script is using the following mart XML
                config file:<br>
                <br>
                <pre><?xml version="1.0" encoding="UTF-8"?>
</pre>
                <pre><!DOCTYPE MartRegistry>
</pre>
                <pre><MartRegistry>
</pre>
                <pre>    <MartURLLocation
</pre>
                <pre>        name         = "ensembl"
</pre>
                <pre>        displayName  = "ensembl"
</pre>
                <pre>        host         = "<a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="http://www.biomart.org/">www.biomart.org</a>"
</pre>
                <pre>        port         = "80"
</pre>
                <pre>        visible      = "1"
</pre>
                <pre>        default      = ""
</pre>
                <pre>        includeDatasets = "hsapiens_gene_ensembl"
</pre>
                <pre>        martUser     = ""
</pre>
                <pre>    />
</pre>
                <pre></MartRegistry>

</pre>
                However, I'm getting BioMart queries from hg19 and not
                hg38. Does the default port '80' still working on hg19?
                how can I specify I would like to use hg38 biomart
                service.<br>
                <br>
                On the other hand, it's always useful to know how to
                query older Ensembl versions with Biomart. However using
                $action=clean with conf file of ensembl 75 archive it's
                leading me to massive amounts of RAM consumption in my
                Perl script.<br>
                <br>
                Thanks.<br>
                <br>
                Best regards,<br>
                Guillermo.<br>
                <div class="moz-cite-prefix">On 19/09/14 15:48, Thomas
                  Maurel wrote:<br>
                </div>
                <blockquote cite="mid:4B2EFDEF-33F9-4F59-8B37-30795F2DA7CA@ebi.ac.uk" type="cite">
                  <meta http-equiv="Content-Type" content="text/html;
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                  Dear Guillermo,
                  <div><br>
                  </div>
                  <div>If you are using:</div>
                  <div>1) the biomart-perl/scripts/webExample.pl script
                    and an xml file</div>
                  <div>You can change the path to the biomart website in
                    the following line:</div>
                  <blockquote style="margin: 0 0 0 40px; border: none;
                    padding: 0px;">
                    <div>my $path="<a moz-do-not-send="true" href="http://www.biomart.org/biomart/martservice?">http://www.biomart.org/biomart/martservice?</a>";</div>
                  </blockquote>
                  <div>With the path to our Ensembl release 75 archive:</div>
                  <blockquote style="margin: 0 0 0 40px; border: none;
                    padding: 0px;">
                    <div>my $path="<a moz-do-not-send="true" href="http://feb2014.archive.ensembl.org/biomart/martservice?">http://feb2014.archive.ensembl.org/biomart/martservice?</a>";</div>
                  </blockquote>
                  <div><br>
                  </div>
                  2) A BioMart perl script
                  <div>a) You first need to edit your configuration file
                    in "biomart-perl/conf/martURLLocation.xml" and paste
                    the content of the mart registry page for the
                    Ensembl release 75 archive website: <a moz-do-not-send="true" href="http://feb2014.archive.ensembl.org/biomart/martservice?type=registry">http://feb2014.archive.ensembl.org/biomart/martservice?type=registry</a></div>
                  <div>b) Then edit your script and make sure that "my
                    $confFile" variable is looking at
                    the martURLLocation.xml configuration file in
                    biomart-perl/conf</div>
                  <div>c) Finally, make sure to update the following
                    line in your script:</div>
                  <div><br>
                  </div>
                  <div>my $action='cached';</div>
                  <div>
                    <div><br>
                    </div>
                    <div>with:</div>
                    <div><br>
                    </div>
                    <div>my $action='clean';</div>
                    <div>
                      <div>
                        <div><br>
                        </div>
                        <div>The first run of your script on Ensembl 75
                          might be a bit slow as BioMart will cache some
                          data from the BioMart website.</div>
                        <div>Once you have run your script with the
                          action variable set to "clean", you can set
                          the variable to "cached" again.</div>
                        <div><br>
                        </div>
                        <div>Hope this helps,</div>
                        <div>Best regards,</div>
                        <div>Thomas</div>
                        <div>On 19 Sep 2014, at 13:28, Guillermo Marco
                          Puche <<a moz-do-not-send="true" href="mailto:guillermo.marco@sistemasgenomicos.com">guillermo.marco@sistemasgenomicos.com</a>>


                          wrote:</div>
                        <br class="Apple-interchange-newline">
                        <blockquote type="cite">
                          <meta http-equiv="content-type" content="text/html; charset=windows-1252">
                          <div bgcolor="#FFFFFF" text="#000066"> Dear
                            developers,<br>
                            <br>
                            I would like to know how to specify BioMart
                            Perl code to query against older Ensembl
                            version (ie:75) and not latest (I believe
                            used by default).<br>
                            <br>
                            Thank you.<br>
                            <br>
                            Best regards,<br>
                            Guillermo.<br>
                          </div>
_______________________________________________<br>
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                          Ensembl Blog: <a moz-do-not-send="true" href="http://www.ensembl.info/">http://www.ensembl.info/</a><br>
                        </blockquote>
                      </div>
                      <br>
                      <div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;">
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                              <div style="word-wrap: break-word;
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                                -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate;
                                  font-family: Helvetica; font-style:
                                  normal; font-variant: normal;
                                  font-weight: normal; letter-spacing:
                                  normal; line-height: normal; orphans:
                                  2; text-indent: 0px; text-transform:
                                  none; white-space: normal; widows: 2;
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                                  0px;
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                                  none; -webkit-text-stroke-width: 0px;">
                                  <div style="word-wrap: break-word;
                                    -webkit-nbsp-mode: space;
                                    -webkit-line-break:
                                    after-white-space; "><span class="Apple-style-span" style="border-collapse: separate;
                                      font-family: Helvetica;
                                      font-style: normal; font-variant:
                                      normal; font-weight: normal;
                                      letter-spacing: normal;
                                      line-height: normal; orphans: 2;
                                      text-indent: 0px; text-transform:
                                      none; white-space: normal; widows:
                                      2; word-spacing: 0px;
                                      border-spacing: 0px;
                                      -webkit-text-decorations-in-effect:
                                      none; -webkit-text-stroke-width:
                                      0px;">
                                      <div style="word-wrap: break-word;
                                        -webkit-nbsp-mode: space;
                                        -webkit-line-break:
                                        after-white-space; ">
                                        <div>--</div>
                                        <div>Thomas Maurel<br>
                                          Bioinformatician - Ensembl
                                          Production Team<br>
                                          European Bioinformatics
                                          Institute (EMBL-EBI)<br>
                                          European Molecular Biology
                                          Laboratory<br>
                                          Wellcome Trust Genome Campus<br>
                                          Hinxton<br>
                                          Cambridge CB10 1SD<br>
                                          United Kingdom</div>
                                      </div>
                                    </span></div>
                                </span></div>
                            </span></div>
                        </span> </div>
                      <br>
                    </div>
                  </div>
                  <br>
                  <fieldset class="mimeAttachmentHeader"></fieldset>
                  <br>
                  <pre wrap="">_______________________________________________
Dev mailing list    <a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="mailto:Dev@ensembl.org">Dev@ensembl.org</a>
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Ensembl Blog: <a moz-do-not-send="true" class="moz-txt-link-freetext" href="http://www.ensembl.info/">http://www.ensembl.info/</a>
</pre>
                </blockquote>
              </div>
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          </div>
          <br>
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                        after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-stroke-width: 0px;">
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                            <div>--</div>
                            <div>Thomas Maurel<br>
                              Bioinformatician - Ensembl Production Team<br>
                              European Bioinformatics Institute
                              (EMBL-EBI)<br>
                              European Molecular Biology Laboratory<br>
                              Wellcome Trust Genome Campus<br>
                              Hinxton<br>
                              Cambridge CB10 1SD<br>
                              United Kingdom</div>
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        <pre wrap="">_______________________________________________
Dev mailing list    <a moz-do-not-send="true" class="moz-txt-link-abbreviated" href="mailto:Dev@ensembl.org">Dev@ensembl.org</a>
Posting guidelines and subscribe/unsubscribe info: <a moz-do-not-send="true" class="moz-txt-link-freetext" href="http://lists.ensembl.org/mailman/listinfo/dev">http://lists.ensembl.org/mailman/listinfo/dev</a>
Ensembl Blog: <a moz-do-not-send="true" class="moz-txt-link-freetext" href="http://www.ensembl.info/">http://www.ensembl.info/</a>
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      <pre wrap="">_______________________________________________
Dev mailing list    <a class="moz-txt-link-abbreviated" href="mailto:Dev@ensembl.org">Dev@ensembl.org</a>
Posting guidelines and subscribe/unsubscribe info: <a class="moz-txt-link-freetext" href="http://lists.ensembl.org/mailman/listinfo/dev">http://lists.ensembl.org/mailman/listinfo/dev</a>
Ensembl Blog: <a class="moz-txt-link-freetext" href="http://www.ensembl.info/">http://www.ensembl.info/</a>
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_______________________________________________<br>Dev mailing list    <a href="mailto:Dev@ensembl.org">Dev@ensembl.org</a><br>Posting guidelines and subscribe/unsubscribe info: <a href="http://lists.ensembl.org/mailman/listinfo/dev">http://lists.ensembl.org/mailman/listinfo/dev</a><br>Ensembl Blog: <a href="http://www.ensembl.info/">http://www.ensembl.info/</a><br></blockquote></div><br><div>
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;  "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;  "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;  "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;  "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>--</div><div>Thomas Maurel<br>Bioinformatician - Ensembl Production Team<br>European Bioinformatics Institute (EMBL-EBI)<br>European Molecular Biology Laboratory<br>Wellcome Trust Genome Campus<br>Hinxton<br>Cambridge CB10 1SD<br>United Kingdom</div></div></span></div></span></div></span></div></span>
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