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Dear all,<br>
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I am working on a specie whose reference genome is not publicly
available. I have a .gtf file for CDS annotations and a fasta file
for the genome sequence.<br>
<br>
I am using gtf2vep.pl to generate my .vep file from my .vcf file.<br>
<br>
I would like to obtain the transcript nucleotide sequences of my
CDSs ( coding sequences of my genes without UTRs...).<br>
Is there a way to connect to the cache file generated with
gtf2vep.pl (instead of the database registry) and use Ensembl API
objects to get the coding sequences of my genes?<br>
<br>
Thanks in advance for your answer.<br>
<br>
Best regards,<br>
<br>
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