<div dir="ltr">Hi Caleb,<div><br></div><div>Your input to the POST endpoint indicates your variant is on the reverse strand; the "-" in the 5th column tells VEP this. Remove this or replace with "+" and the answers match OK. See [1] for details.</div><div><br></div><div>Your HGVS input to the GET endpoint is implicitly on the forward strand.</div><div><br></div><div>Regards</div><div><br></div><div>Will McLaren</div><div>Ensembl Variation</div><div><br></div><div>[1] : <a href="http://www.ensembl.org/info/docs/tools/vep/vep_formats.html#input">http://www.ensembl.org/info/docs/tools/vep/vep_formats.html#input</a></div><div class="gmail_extra"><br><div class="gmail_quote">On 11 July 2016 at 16:04, Caleb Davis <span dir="ltr"><<a href="mailto:caldavis@gmail.com" target="_blank">caldavis@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div dir="ltr"><div>Hi dev-ensemble,</div><div><br></div><div>I'm a happy user of your code and services. Thank you for all you do.</div><div><br></div><div>I noticed a consistent difference between POST and GET commands at the vep/human/region endpoint of the v84 API (GRCh37). Specifically, the variant allele differs for transcripts on the '-' strand.</div><div><br></div><div>e.g. 17 41240915 41240915 A/G - (vep default). </div><div><br></div><div>Using POST [1] hgvsc is, e.g. ENST00000346315.3:c.4185+2046T>G (notice T>G; not reverse complemented)</div><div><br></div><div>Using GET [2] hgvsc is, e.g. <font color="#000000"><span style="white-space:pre-wrap">ENST00000346315.3:c.4185+2046T>C (T>C; reverse complemented)</span></font><br></div><div><br></div><div><a href="http://grch37.rest.ensembl.org/vep/human/hgvs/17:g.41240915A%3EG?hgvs&content-type=application/json" target="_blank"></a>Would you please take a look? Thanks in advance.</div><div><br></div><div>-- Caleb<div><div data-smartmail="gmail_signature">Caleb Davis, PhD</div><div data-smartmail="gmail_signature">Bioinformatics Scientist</div><div data-smartmail="gmail_signature">MolecularMatch, Inc.</div><div data-smartmail="gmail_signature">twitter: @cal_dav</div></div><div data-smartmail="gmail_signature"><br></div><div>[1] curl -X POST -H "content-type: application/json" -H "accept: application/json" -H "Cache-Control: no-cache" -H "Postman-Token: f224211e-6267-22cf-5a11-65f2cf00631d" -d '{ "variants" : ["17 41240915 41240915 A/G -"], "numbers":"1", "hgvs":"1" }' "<a href="http://grch37.rest.ensembl.org/vep/human/region" target="_blank">http://grch37.rest.ensembl.org/vep/human/region</a>"<br></div><div><br></div><div>[2] <a href="http://grch37.rest.ensembl.org/vep/human/hgvs/17:g.41240915A%3EG?hgvs&content-type=application/json" target="_blank">http://grch37.rest.ensembl.org/vep/human/hgvs/17:g.41240915A%3EG?hgvs&content-type=application/json</a></div>
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