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<p>Hello, </p>
<p><br>
</p>
<p>I am searching the human GRCh38.p10 through ensembl. For example I search the location of the PTEN gene according to the locations given by the NIH site:
<a href="https://ghr.nlm.nih.gov/gene/PTEN#sourcesforpage" class="OWAAutoLink" id="LPlnk294406" previewremoved="true">
https://ghr.nlm.nih.gov/gene/PTEN#sourcesforpage</a></p>
<br>
<p>According<font size="3"> to that site, the PTEN location is 10:</font><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 12pt;">87,863,438-87,971,930</span><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;"><font size="3">. </font></span></p>
<p><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;"><font size="3"><br>
</font></span></p>
<p><font size="3"><font color="#202020" face="Roboto, Helvetica Neue, Helvetica, Arial, sans-serif">I then entered the above location onto the ensembl GRCh38.p10 browser but changed the first number to </font></font><font color="#202020" face="Roboto, Helvetica Neue, Helvetica, Arial, sans-serif" size="3">87,763,438
to move more </font><font color="#202020" face="Roboto, Helvetica Neue, Helvetica, Arial, sans-serif">upstream</font><font color="#202020" face="Roboto, Helvetica Neue, Helvetica, Arial, sans-serif" size="3">. Within the new region of </font><font size="3">10:</font><span style="font-size: 12pt; color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;">87,763,438-87,971,930</span><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;"><font size="3">,
the sequence AGGTCA should be found but it is not revealed on the ensembl. </font></span></p>
<p><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;"><font size="3"><br>
</font></span></p>
<p><span style="color: rgb(32, 32, 32); font-family: Roboto, 'Helvetica Neue', Helvetica, Arial, sans-serif;"><font size="3">Brad</font></span></p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Dev <dev-bounces@ensembl.org> on behalf of Ben Moore <bmoore@ebi.ac.uk><br>
<b>Sent:</b> June 6, 2017 10:21:31 AM<br>
<b>To:</b> Ensembl developers list<br>
<b>Subject:</b> Re: [ensembl-dev] Ensemble Search Help</font>
<div> </div>
</div>
<div>Hi Brad,
<div class=""><br class="">
</div>
<div class="">We can definitely try and help you. You can get help through this mailing list, or you can send further information to
<a href="mailto:helpdesk@ensembl.org" class="">helpdesk@ensembl.org</a>, which will create a ticket in our helpdesk system, where a member of the Ensembl team will help you directly. In any case, we’d need to know some more information:</div>
<div class="">- which species are you searching? which genome assembly are you using in your work?</div>
<div class="">- do you have an example set of genomic coordinates that you have used?</div>
<div class="">- what do you see (screenshot)? What do you expect to see?</div>
<div class=""><br class="">
</div>
<div class="">Best wishes</div>
<div class=""><br class="">
</div>
<div class="">Ben</div>
<div class=""><br class="">
<div>
<blockquote type="cite" class="">
<div class="">On 6 Jun 2017, at 15:15, Bradley Ross <<a href="mailto:1br6@queensu.ca" class="">1br6@queensu.ca</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div id="divtagdefaultwrapper" dir="ltr" style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif;" class="">
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class="">Hello,</font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class=""><br class="">
</font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class="">I was wondering if there was someone I could contact regarding using the genome browser Ensembl. </font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class=""><br class="">
</font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class="">I have searched specific regions where I know a particular sequence should be located, but it does not come up when I search through this genome browser. </font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class=""><br class="">
</font></div>
<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class="">Any assistance would be greatly appreciated. </font></div>
<div class=""><br class="">
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<div class=""><font face="Calibri,Arial,Helvetica,sans-serif,serif,EmojiFont" class="">Brad</font></div>
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<span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">Dev
mailing list </span><a href="mailto:Dev@ensembl.org" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">Dev@ensembl.org</a><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">Posting
guidelines and subscribe/unsubscribe info:<span class="Apple-converted-space"> </span></span><a href="http://lists.ensembl.org/mailman/listinfo/dev" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">http://lists.ensembl.org/mailman/listinfo/dev</a><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">Ensembl
Blog:<span class="Apple-converted-space"> </span></span><a href="http://www.ensembl.info/" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">http://www.ensembl.info/</a></div>
</blockquote>
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<br class="">
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<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div class="">Ben Moore</div>
<div class="">Ensembl Outreach Officer</div>
<div class=""><br class="">
</div>
<div class="">European Bioinformatics Institute (EMBL-EBI)</div>
<div class="">European Molecular Biology Laboratory</div>
<div class="">Wellcome Trust Genome Campus</div>
<div class="">Hinxton</div>
<div class="">Cambridge</div>
<div class="">CB10 1SD</div>
<div class="">UK</div>
<div class=""><br class="">
</div>
<div class=""><a href="mailto:bmoore@ebi.ac.uk" class="">bmoore@ebi.ac.uk</a></div>
<div class="">+44 (0)1223 494265</div>
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