<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Pankaj<div class="">Which Ensembl release did you use for your analyses?</div><div class=""><br class=""><div class="">In the latest release, e89, there is around 20,000 protein coding genes in the human genome and almost 40,000 pseudogenes and non coding genes.</div><div class=""><br class=""></div><div class="">Thanks</div><div class="">Thibaut</div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On 7 Jun 2017, at 16:26, Pankaj Agarwal <<a href="mailto:p.agarwal@duke.edu" class="">p.agarwal@duke.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Hi,<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">I am using Ensembl human reference genome and annotation for alignment and mapping rna-seq data. In my results I get ~ 60,000 unique ENSG ID. I am wondering why there are so many more than ~ 20,000 genes in the human genome.<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Thanks,<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">- Pankaj<o:p class=""></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 8pt;" class="">----------------------------------------------------------------<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 8pt;" class="">Pankaj Agarwal, M.S<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 8pt;" class="">Bioinformatician<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; 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