<div dir="ltr"><div class="gmail_default" style="font-family:tahoma,sans-serif">Hello,</div><div class="gmail_default" style="font-family:tahoma,sans-serif">I am using the API on a system which is fine. However, as I tried to build the API on another system, I faced some problems. I think I followed all steps, but my script fails to work. Please see the following output</div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif">mahmood@qemu:~$ echo $PERL5LIB<br>:/home/mahmood/src/bioperl-1.6.1:/home/mahmood/src/ensembl/modules:/home/mahmood/src/ensembl-compara/modules:/home/mahmood/src/ensembl-variation/modules:/home/mahmood/src/ensembl-funcgen/modules:/home/mahmood/src/Bio-DB-HTS/lib:/home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/:/home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/Faidx:/home/mahmood/src/bioperl-1.6.1:/home/mahmood/src/ensembl/modules:/home/mahmood/src/ensembl-compara/modules:/home/mahmood/src/ensembl-variation/modules:/home/mahmood/src/ensembl-funcgen/modules:/home/mahmood/src/Bio-DB-HTS/lib:/home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/:/home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/Faidx<br>mahmood@qemu:~$ echo $HTSLIB_DIR<br>/home/mahmood/src/htslib<br>mahmood@qemu:~$ perl <a href="http://test-new.pl">test-new.pl</a> genes.txt <br>Can't locate DBI.pm in @INC (you may need to install the DBI module) (@INC contains: /home/mahmood/src/bioperl-1.6.1 /home/mahmood/src/ensembl/modules /home/mahmood/src/ensembl-compara/modules /home/mahmood/src/ensembl-variation/modules /home/mahmood/src/ensembl-funcgen/modules /home/mahmood/src/Bio-DB-HTS/lib /home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/ /home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/Faidx /home/mahmood/src/bioperl-1.6.1 /home/mahmood/src/ensembl/modules /home/mahmood/src/ensembl-compara/modules /home/mahmood/src/ensembl-variation/modules /home/mahmood/src/ensembl-funcgen/modules /home/mahmood/src/Bio-DB-HTS/lib /home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/ /home/mahmood/src/Bio-DB-HTS/blib/arch/auto/Bio/DB/HTS/Faidx /etc/perl /usr/local/lib/x86_64-linux-gnu/perl/5.22.1 /usr/local/share/perl/5.22.1 /usr/lib/x86_64-linux-gnu/perl5/5.22 /usr/share/perl5 /usr/lib/x86_64-linux-gnu/perl/5.22 /usr/share/perl/5.22 /usr/local/lib/site_perl /usr/lib/x86_64-linux-gnu/perl-base .) at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm line 74.<br>BEGIN failed--compilation aborted at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm line 74.<br>Compilation failed in require at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm line 69.<br>BEGIN failed--compilation aborted at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm line 69.<br>Compilation failed in require at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/Registry.pm line 137.<br>BEGIN failed--compilation aborted at /home/mahmood/src/ensembl/modules/Bio/EnsEMBL/Registry.pm line 137.<br>Compilation failed in require at <a href="http://test-new.pl">test-new.pl</a> line 1.<br>BEGIN failed--compilation aborted at <a href="http://test-new.pl">test-new.pl</a> line 1.<br>mahmood@qemu:~$ head -n 3 <a href="http://test-new.pl">test-new.pl</a> <br>use Bio::EnsEMBL::Registry;<br>use Bio::SeqIO;<br>use File::Basename;<br>mahmood@qemu:~$ <br></div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif">Which step did I miss? Any thought?<br></div><div class="gmail_default" style="font-family:tahoma,sans-serif"><br clear="all"></div><div><div class="gmail_signature"><div dir="ltr"><font face="tahoma,sans-serif">Regards,<br>Mahmood</font><br><br><br></div></div></div>
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