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    <p>Hello Felix</p>
    <p>You could use the ensembl REST server (<a class="moz-txt-link-freetext" href="http://rest.ensembl.org/">http://rest.ensembl.org/</a>)</p>
    To retrieve the list of available species and genome builds, use the
    info/species endpoint: <br>
    <a class="moz-txt-link-freetext" href="http://rest.ensembl.org/info/species?content-type=application/json">http://rest.ensembl.org/info/species?content-type=application/json</a><br>
    <br>
    To retrieve the sequence information, use the sequence endpoint:<br>
    <br>
    <a class="moz-txt-link-freetext" href="http://rest.ensembl.org/documentation/info/sequence_region_post">http://rest.ensembl.org/documentation/info/sequence_region_post</a>
    (Request multiple types of sequence by a list of regions)<br>
    <br>
    <a class="moz-txt-link-freetext" href="http://rest.ensembl.org/documentation/info/sequence_region">http://rest.ensembl.org/documentation/info/sequence_region</a> (Returns
    the genomic sequence of the specified region of the given species)<br>
    <br>
    Please be aware that there is a rate limit (15 requests per
    second)   <br>
    <br>
    More info in User Guide:<br>
    <a class="moz-txt-link-freetext" href="https://github.com/Ensembl/ensembl-rest/wiki">https://github.com/Ensembl/ensembl-rest/wiki</a><br>
    <a class="moz-txt-link-freetext" href="https://github.com/Ensembl/ensembl-rest/wiki/Rate-Limits">https://github.com/Ensembl/ensembl-rest/wiki/Rate-Limits</a><br>
    <br>
    Hope it helps,<br>
    <br>
    Best Regards<br>
    Prem<br>
    <br>
    <div class="moz-cite-prefix">On 03/01/2018 20:24, Felix Krueger
      wrote:<br>
    </div>
    <blockquote type="cite"
cite="mid:EFD89DE93ECF2C4E98CA6FB9C079B86A0313974A71@MAIL2.babraham.ac.uk">
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          Dear all,</p>
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          I am looking to use the Ensembl API to retrieve lists of
          available species and genome builds, as well as potentially
          large numbers of sequence information for sets of coordinates
          of various genomes. For this Java-based project we cannot use
          the Perl API, but could potentially use the REST or direct
          MySQL access. Would anyone be able to give me a pointer to
          what is likely the best way for me going forward?</p>
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        <p class="MsoNormal" style="margin: 0cm 0cm 0.0001pt; font-size:
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          Many thanks and a Happy New Year!</p>
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          11pt; font-family: Calibri, sans-serif;">
          Kind regards,</p>
        <p class="MsoNormal" style="margin: 0cm 0cm 0.0001pt; font-size:
          11pt; font-family: Calibri, sans-serif;">
          Felix</p>
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          11pt; font-family: Calibri, sans-serif;">
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      <pre wrap="">_______________________________________________
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</pre>
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