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    <p>I would suggest setting the first release to the ensembl code
      version that you are using.<br>
    </p>
    <br>
    <div class="moz-cite-prefix">On 15/01/2018 16:22, Francesco Lamanna
      wrote:<br>
    </div>
    <blockquote type="cite"
cite="mid:CAM_GrBHhnbiwQ-XMnJ0Y9d=hRhqOd294xbKJbn4XqV-Cp335Ew@mail.gmail.com">
      <div dir="ltr">
        <div>
          <div>Hi Wasiu,<br>
            <br>
          </div>
          this is the output of the species_set_header table:<br>
          <br>
          mysql> SELECT * FROM species_set_header;<br>
+----------------+-------------------------------+------+---------------+--------------+<br>
          | species_set_id | name                          | size |
          first_release | last_release |<br>
+----------------+-------------------------------+------+---------------+--------------+<br>
          |              1 | H.sap-G.gal                   |    2
          |          NULL |         NULL |<br>
          |              2 | H.sap-B.flo                   |    2
          |          NULL |         NULL |<br>
          |              3 | H.sap-P.mar                   |    2
          |          NULL |         NULL |<br>
          |              4 | <a href="http://H.sap-C.mil"
            moz-do-not-send="true">H.sap-C.mil</a>                  
          |    2 |          NULL |         NULL |<br>
          |              5 | G.gal-B.flo                   |    2
          |          NULL |         NULL |<br>
          |              6 | G.gal-P.mar                   |    2
          |          NULL |         NULL |<br>
          |              7 | <a href="http://G.gal-C.mil"
            moz-do-not-send="true">G.gal-C.mil</a>                  
          |    2 |          NULL |         NULL |<br>
          |              8 | B.flo-P.mar                   |    2
          |          NULL |         NULL |<br>
          |              9 | <a href="http://B.flo-C.mil"
            moz-do-not-send="true">B.flo-C.mil</a>                  
          |    2 |          NULL |         NULL |<br>
          |             10 | <a href="http://P.mar-C.mil"
            moz-do-not-send="true">P.mar-C.mil</a>                  
          |    2 |          NULL |         NULL |<br>
          |             11 | H.sap                         |    1
          |          NULL |         NULL |<br>
          |             12 | G.gal                         |    1
          |          NULL |         NULL |<br>
          |             13 | B.flo                         |    1
          |          NULL |         NULL |<br>
          |             14 | P.mar                         |    1
          |          NULL |         NULL |<br>
          |             15 | C.mil                         |    1
          |          NULL |         NULL |<br>
          |             16 | <a
            href="http://H.sap-G.gal-B.flo-P.mar-C.mil"
            moz-do-not-send="true">H.sap-G.gal-B.flo-P.mar-C.mil</a>
          |    5 |          NULL |         NULL |<br>
+----------------+-------------------------------+------+---------------+--------------+<br>
          16 rows in set (0.05 sec)<br>
          <br>
        </div>
        It looks ok<br>
      </div>
      <div class="gmail_extra"><br>
        <div class="gmail_quote">2018-01-15 17:19 GMT+01:00 Wasiu Akanni
          <span dir="ltr"><<a href="mailto:waakanni@ebi.ac.uk"
              target="_blank" moz-do-not-send="true">waakanni@ebi.ac.uk</a>></span>:<br>
          <blockquote class="gmail_quote" style="margin:0 0 0
            .8ex;border-left:1px #ccc solid;padding-left:1ex">
            <div text="#000000" bgcolor="#FFFFFF">
              <p>Hi Francesco,</p>
              <p>Have you checked the species_set_header table?<br>
              </p>
              <div>
                <div class="h5"> <br>
                  <div class="m_-2801295335211067424moz-cite-prefix">On
                    15/01/2018 14:39, Francesco Lamanna wrote:<br>
                  </div>
                  <blockquote type="cite">
                    <div dir="ltr">
                      <div>
                        <div>
                          <div>
                            <div>
                              <div>
                                <div>Hi Matthieu,<br>
                                  <br>
                                </div>
                                thank you for your suggestion, it fixed
                                the problem.<br>
                                <br>
                              </div>
                              However, I still get the following error:<br>
                              <br>
                              mysql> SELECT * FROM msg;<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>--------------------+---------<wbr>-+<br>
                              | analysis_id |
                              logic_name                    |
                              log_message_id | job_id | role_id |
                              worker_id | when_logged         | retry |
                              status       |
                              msg                           <wbr>                            
                              | is_error |<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>--------------------+---------<wbr>-+<br>
                              |           9 |
                              copy_ncbi_table              
                              |              1 |      5 |       3
                              |         3 | 2018-01-15 14:49:29 |     0
                              | WRITE_OUTPUT | Successfully copied
                              1646504 'ncbi_taxa_node' rows         
                              |        0 |<br>
                              |           9 |
                              copy_ncbi_table              
                              |              2 |      6 |       4
                              |         4 | 2018-01-15 14:50:36 |     0
                              | WRITE_OUTPUT | Successfully copied
                              2504391 'ncbi_taxa_name' rows         
                              |        0 |<br>
                              |          10 |
                              populate_method_links_from_db
                              |              3 |      7 |       6
                              |         6 | 2018-01-15 14:52:06 |     0
                              | WRITE_OUTPUT | Successfully copied 19
                              'method_link' rows                 
                              |        0 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              4 |     10 |       9
                              |         9 | 2018-01-15 14:55:10 |     0
                              | WRITE_OUTPUT | The  species-set could
                              not be found in the master database
                              |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              5 |     10 |      10
                              |        10 | 2018-01-15 14:56:11 |     1
                              | WRITE_OUTPUT | The  species-set could
                              not be found in the master database
                              |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              6 |     10 |      11
                              |        11 | 2018-01-15 14:57:12 |     2
                              | WRITE_OUTPUT | The  species-set could
                              not be found in the master database
                              |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              7 |     10 |      12
                              |        12 | 2018-01-15 14:58:21 |     3
                              | WRITE_OUTPUT | The  species-set could
                              not be found in the master database
                              |        1 |<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>--------------------+---------<wbr>-+<br>
                              7 rows in set (0.01 sec)<br>
                              <br>
                            </div>
                            But my species_set in the master_db is not
                            empty:<br>
                            <br>
                            mysql> SELECT * FROM species_set;<br>
                            +----------------+------------<wbr>--+<br>
                            | species_set_id | genome_db_id |<br>
                            +----------------+------------<wbr>--+<br>
                            |              1 |            1 |<br>
                            |              1 |            2 |<br>
                            |              2 |            1 |<br>
                            |              2 |            3 |<br>
                            |              3 |            1 |<br>
                            |              3 |            4 |<br>
                            |              4 |            1 |<br>
                            |              4 |            5 |<br>
                            |              5 |            2 |<br>
                            |              5 |            3 |<br>
                            |              6 |            2 |<br>
                            |              6 |            4 |<br>
                            |              7 |            2 |<br>
                            |              7 |            5 |<br>
                            |              8 |            3 |<br>
                            |              8 |            4 |<br>
                            |              9 |            3 |<br>
                            |              9 |            5 |<br>
                            |             10 |            4 |<br>
                            |             10 |            5 |<br>
                            |             11 |            1 |<br>
                            |             12 |            2 |<br>
                            |             13 |            3 |<br>
                            |             14 |            4 |<br>
                            |             15 |            5 |<br>
                            |             16 |            1 |<br>
                            |             16 |            2 |<br>
                            |             16 |            3 |<br>
                            |             16 |            4 |<br>
                            |             16 |            5 |<br>
                            +----------------+------------<wbr>--+<br>
                            30 rows in set (0.00 sec)<br>
                            <br>
                          </div>
                          I am quite puzzled by this error.<br>
                          <br>
                        </div>
                        Cheers,<br>
                      </div>
                      Francesco.<br>
                    </div>
                    <div class="gmail_extra"><br>
                      <div class="gmail_quote">2018-01-12 17:48
                        GMT+01:00 Matthieu Muffato <span dir="ltr"><<a
                            href="mailto:muffato@ebi.ac.uk"
                            target="_blank" moz-do-not-send="true">muffato@ebi.ac.uk</a>></span>:<br>
                        <blockquote class="gmail_quote" style="margin:0
                          0 0 .8ex;border-left:1px #ccc
                          solid;padding-left:1ex">Hi Francesco<br>
                          <br>
                          Homoeologues are only used when running on
                          plant genomes (which have polyploid genomes),
                          but the pipeline configuration is shared and
                          expects this method_link to be present<br>
                          <br>
                          This how it looks in the Ensembl Plants
                          database. You can insert this row in your
                          database and it should work<br>
                          <br>
                          <a
href="http://ensro@mysql-eg-publicsql.ebi.ac.uk:4157/ensembl_compara_plants_38_91"
                            rel="noreferrer" target="_blank"
                            moz-do-not-send="true">ensro@mysql-eg-publicsql.ebi.a<wbr>c.uk:4157/ensembl_compara_plan<wbr>ts_38_91</a>
                          [Fri Jan 12 16:46:32 2018] > SELECT * FROM
                          method_link WHERE method_link_id = 206;<br>
                          +----------------+------------<wbr>----------+-------------------<wbr>+<br>
                          | method_link_id | type                 |
                          class             |<br>
                          +----------------+------------<wbr>----------+-------------------<wbr>+<br>
                          |            206 | ENSEMBL_HOMOEOLOGUES |
                          Homology.homology |<br>
                          +----------------+------------<wbr>----------+-------------------<wbr>+<br>
                          <br>
                          Regards,<br>
                          Matthieu<span><br>
                            <br>
                            On 12/01/18 14:01, Francesco Lamanna wrote:<br>
                          </span>
                          <blockquote class="gmail_quote"
                            style="margin:0 0 0 .8ex;border-left:1px
                            #ccc solid;padding-left:1ex"><span> Hi
                              Mateus,<br>
                              <br>
                              I could solve this problem by commenting
                              out the line: "die "The master dabase must
                              be defined with a collection" if
                              $self->o('master_db') and not
                              $self->o('collection');"<br>
                              <br>
                              in LoadMembers_conf.pm. The member_db is
                              now correctly set up.<br>
                              <br>
                              However, when I run the Protein trees
                              pipeline I get the following error message<br>
                              <br>
                              mysql> SELECT * FROM msg;<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-----------------+----------+<br>
                              | analysis_id |
                              logic_name                    |
                              log_message_id | job_id | role_id |
                              worker_id | when_logged         | retry |
                              status       | msg                       
                                                                       
                                                                       
                                                                       
                                                     | is_error |<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-----------------+----------+<br>
                              |           9 |
                              copy_ncbi_table              
                              |              1 |      5 |       3
                              |         3 | 2018-01-12 14:21:39 |     0
                              | WRITE_OUTPUT | Successfully copied
                              1646504 'ncbi_taxa_node' rows             
                                                                       
                                                                       
                                                           |        0 |<br>
                              |           9 |
                              copy_ncbi_table              
                              |              2 |      6 |       4
                              |         4 | 2018-01-12 14:25:27 |     0
                              | WRITE_OUTPUT | Successfully copied
                              2504391 'ncbi_taxa_name' rows             
                                                                       
                                                                       
                                                           |        0 |<br>
                              |          10 |
                              populate_method_links_from_db
                              |              3 |      7 |       6
                              |         6 | 2018-01-12 14:26:54 |     0
                              | WRITE_OUTPUT | Successfully copied 18
                              'method_link' rows                       
                                                                       
                                                                       
                                                         |        0 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              4 |     10 |       9
                              |         9 | 2018-01-12 14:29:56 |     0
                              | FETCH_INPUT  | Cannot find the
                              method_link 'ENSEMBL_HOMOEOLOGUES' at
                              /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Pre<wbr>pareSpeciesSetsMLSS.pm
                              line 70. |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              5 |     10 |      10
                              |        10 | 2018-01-12 14:30:57 |     1
                              | FETCH_INPUT  | Cannot find the
                              method_link 'ENSEMBL_HOMOEOLOGUES' at
                              /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Pre<wbr>pareSpeciesSetsMLSS.pm
                              line 70. |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              6 |     10 |      11
                              |        11 | 2018-01-12 14:31:58 |     2
                              | FETCH_INPUT  | Cannot find the
                              method_link 'ENSEMBL_HOMOEOLOGUES' at
                              /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Pre<wbr>pareSpeciesSetsMLSS.pm
                              line 70. |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              7 |     10 |      12
                              |        12 | 2018-01-12 14:33:00 |     3
                              | FETCH_INPUT  | Cannot find the
                              method_link 'ENSEMBL_HOMOEOLOGUES' at
                              /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Pre<wbr>pareSpeciesSetsMLSS.pm
                              line 70. |        1 |<br>
                              |          16 |
                              create_mlss_ss               
                              |              8 |     10 |      13
                              |        13 | 2018-01-12 14:38:36 |     1
                              | FETCH_INPUT  | Cannot find the
                              method_link 'ENSEMBL_HOMOEOLOGUES' at
                              /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Pre<wbr>pareSpeciesSetsMLSS.pm
                              line 70. |        1 |<br>
                              +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-----------------+----------+<br>
                              8 rows in set (0.01 sec)<br>
                              <br>
                              I wasn't aware about this method_link.<br>
                              <br>
                              Do you know how can I fix this?<br>
                              <br>
                              Thanks,<br>
                              Francesco.<br>
                              <br>
                            </span> 2018-01-11 13:28 GMT+01:00 Francesco
                            Lamanna <<a
                              href="mailto:francesco.lamanna@gmail.com"
                              target="_blank" moz-do-not-send="true">francesco.lamanna@gmail.com</a>
                            <mailto:<a
                              href="mailto:francesco.lamanna@gmail.com"
                              target="_blank" moz-do-not-send="true">francesco.lamanna@gmai<wbr>l.com</a>>>:<span><br>
                              <br>
                                  Hi Mateus,<br>
                              <br>
                                  if I try to initialize the
                              LoadMemebers pipeline without<br>
                                  “--collection ensembl”, I get the
                              following error:<br>
                              <br>
                                  The following options are missing:<br>
                                       {'collection'}<br>
                              <br>
                                  I also tried to set 'collection' =>
                              undef, in the conf file, but I<br>
                                  get another error:<br>
                              <br>
                                  The master dabase must be defined with
                              a collection at<br>
                                  /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/PipeConfig/Loa<wbr>dMembers_conf.pm<br>
                                  line 190.<br>
                              <br>
                                  Cheers,<br>
                                  Francesco<br>
                              <br>
                                  2018-01-11 12:01 GMT+01:00 Mateus
                              Patricio <<a
                                href="mailto:mateus@ebi.ac.uk"
                                target="_blank" moz-do-not-send="true">mateus@ebi.ac.uk</a><br>
                            </span>     <mailto:<a
                              href="mailto:mateus@ebi.ac.uk"
                              target="_blank" moz-do-not-send="true">mateus@ebi.ac.uk</a>>>:<span><br>
                              <br>
                                      Hi Francesco,<br>
                              <br>
                                      In your case the solution should
                              be starting the LoadMembers<br>
                                      without the option “--collection
                              ensembl”.<br>
                              <br>
                                      If you start the pipeline without
                              it, it should use all the<br>
                                      current species in your master
                              database.<br>
                              <br>
                                      In Ensembl we have different
                              collections that are used for<br>
                                      different purposes, and the
                              default one is “ensembl”.<br>
                              <br>
                                      Please let me know if this works.<br>
                              <br>
                                      Cheers,<br>
                              <br>
                                      Mateus.<br>
                              <br>
                              <br>
                            </span>
                            <blockquote class="gmail_quote"
                              style="margin:0 0 0 .8ex;border-left:1px
                              #ccc solid;padding-left:1ex"><span>      
                                  On 11 Jan 2018, at 10:13, Francesco
                                Lamanna<br>
                                        <<a
                                  href="mailto:francesco.lamanna@gmail.com"
                                  target="_blank" moz-do-not-send="true">francesco.lamanna@gmail.com</a><br>
                              </span>
                              <div>
                                <div class="m_-2801295335211067424h5">  
                                        <mailto:<a
                                    href="mailto:francesco.lamanna@gmail.com"
                                    target="_blank"
                                    moz-do-not-send="true">francesco.lamanna@gmai<wbr>l.com</a>>>
                                  wrote:<br>
                                  <br>
                                          Hi Mateus,<br>
                                  <br>
                                          many thanks for your answer.<br>
                                  <br>
                                          I am trying to launch the
                                  LoadMembers pipeline in order to<br>
                                          make a member_db, but I get
                                  the following error:<br>
                                  <br>
                                          mysql> SELECT * from msg;<br>
                                          +-------------+---------------<wbr>---------+----------------+---<wbr>-----+---------+-----------+--<wbr>-------------------+-------+--<wbr>------------+-----------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-+----------+<br>
                                          | analysis_id |
                                  logic_name             |
                                  log_message_id |<br>
                                          job_id | role_id | worker_id |
                                  when_logged         | retry |<br>
                                          status       | msg
                                                   | is_error |<br>
                                          +-------------+---------------<wbr>---------+----------------+---<wbr>-----+---------+-----------+--<wbr>-------------------+-------+--<wbr>------------+-----------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-+----------+<br>
                                          |           3 |
                                  copy_table_from_master |             
                                  1 |             4 |       3 |        
                                  3 | 2018-01-11 11:04:02 |     0 |<br>
                                          WRITE_OUTPUT | Successfully
                                  copied 1646504 'ncbi_taxa_node'<br>
                                          rows   |        0 |<br>
                                          |           3 |
                                  copy_table_from_master |             
                                  2 |             5 |       4 |        
                                  4 | 2018-01-11 11:05:46 |     0 |<br>
                                          WRITE_OUTPUT | Successfully
                                  copied 2504391 'ncbi_taxa_name'<br>
                                          rows   |        0 |<br>
                                          |           4 |
                                  load_genomedb_factory  |             
                                  3 |             3 |       5 |        
                                  5 | 2018-01-11 11:07:06 |     0 |<br>
                                          FETCH_INPUT  | Could not fetch
                                  collection ss with name=ensembl<br>
                                          at<br>
                                          /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Gen<wbr>omeDBFactory.pm<br>
                                          line 106. |        1 |<br>
                                          |           4 |
                                  load_genomedb_factory  |             
                                  4 |             3 |       6 |        
                                  6 | 2018-01-11 11:08:00 |     1 |<br>
                                          FETCH_INPUT  | Could not fetch
                                  collection ss with name=ensembl<br>
                                          at<br>
                                          /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Gen<wbr>omeDBFactory.pm<br>
                                          line 106. |        1 |<br>
                                          |           4 |
                                  load_genomedb_factory  |             
                                  5 |             3 |       7 |        
                                  7 | 2018-01-11 11:08:25 |     2 |<br>
                                          FETCH_INPUT  | Could not fetch
                                  collection ss with name=ensembl<br>
                                          at<br>
                                          /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Gen<wbr>omeDBFactory.pm<br>
                                          line 106. |        1 |<br>
                                          |           4 |
                                  load_genomedb_factory  |             
                                  6 |             3 |       8 |        
                                  8 | 2018-01-11 11:09:25 |     3 |<br>
                                          FETCH_INPUT  | Could not fetch
                                  collection ss with name=ensembl<br>
                                          at<br>
                                          /home/hd/hd_hd/hd_cc141/EnsEMB<wbr>L/ensembl-compara/modules/Bio/<wbr>EnsEMBL/Compara/RunnableDB/Gen<wbr>omeDBFactory.pm<br>
                                          line 106. |        1 |<br>
                                          +-------------+---------------<wbr>---------+----------------+---<wbr>-----+---------+-----------+--<wbr>-------------------+-------+--<wbr>------------+-----------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>------------------------------<wbr>-+----------+<br>
                                          6<br>
                                  <br>
                                          Cheers,<br>
                                          Francesco.<br>
                                  <br>
                                          2018-01-10 17:31 GMT+01:00
                                  Mateus Patricio <<a
                                    href="mailto:mateus@ebi.ac.uk"
                                    target="_blank"
                                    moz-do-not-send="true">mateus@ebi.ac.uk</a><br>
                                </div>
                              </div>
                                      <mailto:<a
                                href="mailto:mateus@ebi.ac.uk"
                                target="_blank" moz-do-not-send="true">mateus@ebi.ac.uk</a>>>:<span><br>
                                <br>
                                            Hi Francesco<br>
                                <br>
                                            The protein tree pipeline
                                reuses the genes and sequence<br>
                                            members from the 'reuse_db'
                                parameter, which in this case<br>
                                            should point to a members
                                database.<br>
                                <br>
                                            This members database can be
                                created by running the<br>
                                            LoadMembers pipeline.<br>
                                <br>
                                            You can initiate the
                                pipeline with the following command
                                line:<br>
                                <br>
                              </span>             <a
                                href="http://init_pipeline.pl"
                                rel="noreferrer" target="_blank"
                                moz-do-not-send="true">init_pipeline.pl</a>
                              <<a href="http://init_pipeline.pl/"
                                rel="noreferrer" target="_blank"
                                moz-do-not-send="true">http://init_pipeline.pl/</a>><span><br>
                                           
                                Bio::EnsEMBL::Compara::PipeCon<wbr>fig::EBI::Ensembl::LoadMembers<wbr>_conf<br>
                                            --collection ensembl<br>
                                <br>
                                            Then you should point the
                                parameter reuse_db to this<br>
                                            database on your Protein
                                Tree config file.<br>
                                <br>
                                            'reuse_db'   =>
                                'mysql://ensro@host:port/datab<wbr>ase',<br>
                                <br>
                                            Please do let me know if you
                                have further questions.<br>
                                <br>
                                            Cheers,<br>
                                <br>
                                            Mateus.<br>
                                <br>
                                <br>
                              </span>
                              <blockquote class="gmail_quote"
                                style="margin:0 0 0 .8ex;border-left:1px
                                #ccc solid;padding-left:1ex"><span>    
                                          On 10 Jan 2018, at 16:06,
                                  Francesco Lamanna<br>
                                              <<a
                                    href="mailto:francesco.lamanna@gmail.com"
                                    target="_blank"
                                    moz-do-not-send="true">francesco.lamanna@gmail.com</a><br>
                                </span>
                                <div>
                                  <div class="m_-2801295335211067424h5">
                                                <mailto:<a
                                      href="mailto:francesco.lamanna@gmail.com"
                                      target="_blank"
                                      moz-do-not-send="true">francesco.lamanna@gmai<wbr>l.com</a>>>
                                    wrote:<br>
                                    <br>
                                                Hi all,<br>
                                    <br>
                                                when I try to run the
                                    protein tree pipeline (v91) using<br>
                                                the core Human and
                                    Chicken genomes I get the following<br>
                                                error message:<br>
                                    <br>
                                                mysql> SELECT * from
                                    msg;<br>
                                               
                                    +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>--------------+----------+<br>
                                                | analysis_id |
                                    logic_name                    |<br>
                                                log_message_id | job_id
                                    | role_id | worker_id |<br>
                                                when_logged         |
                                    retry | status       | msg<br>
                                                                       |
                                    is_error |<br>
                                               
                                    +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>--------------+----------+<br>
                                                |           9 |
                                    copy_ncbi_table                     
                                        |              1 |      5
                                    |       3 |         4 |<br>
                                                2018-01-10 16:40:13
                                    |     0 | WRITE_OUTPUT |
                                    Successfully<br>
                                                copied 1646504
                                    'ncbi_taxa_node' rows        
                                    |        0 |<br>
                                                |           9 |
                                    copy_ncbi_table                     
                                        |              2 |      6
                                    |       4 |         3 |<br>
                                                2018-01-10 16:42:04
                                    |     0 | WRITE_OUTPUT |
                                    Successfully<br>
                                                copied 2504391
                                    'ncbi_taxa_name' rows        
                                    |        0 |<br>
                                                |          10 |
                                    populate_method_links_from_db<br>
                                                |              3 |     
                                    7 |       6 |         6 |<br>
                                                2018-01-10 16:43:44
                                    |     0 | WRITE_OUTPUT |
                                    Successfully<br>
                                                copied 18 'method_link'
                                    rows                 |        0 |<br>
                                                |          25 |
                                    genome_member_copy                 
                                         |              4 |     13
                                    |      11 |        11 |<br>
                                                2018-01-10 16:47:49
                                    |     0 | FETCH_INPUT  | ParamError:<br>
                                                value for
                                    param_required('reuse_db') is
                                    required and has<br>
                                                to be defined |        1
                                    |<br>
                                                |          25 |
                                    genome_member_copy                 
                                         |              5 |     14
                                    |      12 |        12 |<br>
                                                2018-01-10 16:47:49
                                    |     0 | FETCH_INPUT  | ParamError:<br>
                                                value for
                                    param_required('reuse_db') is
                                    required and has<br>
                                                to be defined |        1
                                    |<br>
                                               
                                    +-------------+---------------<wbr>----------------+-------------<wbr>---+--------+---------+-------<wbr>----+---------------------+---<wbr>----+--------------+----------<wbr>------------------------------<wbr>------------------------------<wbr>--------------+----------+<br>
                                    <br>
                                                I have no clue about
                                    what to put in the 'reuse_db'<br>
                                                parameter (nor could I
                                    find any information in the<br>
                                                compara docs).<br>
                                    <br>
                                                Can anyone please help
                                    me to solve this issue?<br>
                                    <br>
                                                Thanks,<br>
                                                Francesco.<br>
                                               
                                    ______________________________<wbr>_________________<br>
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                          <span class="m_-2801295335211067424HOEnZb"><font
                              color="#888888"> <br>
                              -- <br>
                              Matthieu Muffato, Ph.D.<br>
                              Ensembl Compara and TreeFam Project Leader<br>
                              European Bioinformatics Institute
                              (EMBL-EBI)<br>
                              European Molecular Biology Laboratory<br>
                              Wellcome Trust Genome Campus, Hinxton<br>
                              Cambridge, CB10 1SD, United Kingdom<br>
                              Room  A3-145<br>
                              Phone <a
                                href="tel:%2B%2044%20%280%29%201223%2049%204631"
                                value="+441223494631" target="_blank"
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                                4631</a><br>
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                                href="tel:%2B%2044%20%280%29%201223%2049%204468"
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                                4468</a><br>
                            </font></span></blockquote>
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                      <br>
                    </div>
                    <br>
                    <fieldset
                      class="m_-2801295335211067424mimeAttachmentHeader"></fieldset>
                    <br>
                    <pre>______________________________<wbr>_________________
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</pre>
                  </blockquote>
                  <br>
                </div>
              </div>
              <span class="HOEnZb"><font color="#888888">
                  <div class="m_-2801295335211067424moz-signature">-- <br>
                    <b><big>Wasiu Ajenifuja Akanni</big></b><br>
                    Developer<br>
                    <br>
                    Compara group<br>
                    EMBL-EBI<br>
                    Phone: <a href="tel:+44%201223%20494237"
                      value="+441223494237" target="_blank"
                      moz-do-not-send="true">+ 44 (0) 1223 494 237</a><br>
                    Room A3145, West building | Wellcome Trust Genome
                    Campus | Hinxton | Cambridge | CB10 1SD | UK</div>
                </font></span></div>
            <br>
            ______________________________<wbr>_________________<br>
            Dev mailing list    <a href="mailto:Dev@ensembl.org"
              moz-do-not-send="true">Dev@ensembl.org</a><br>
            Posting guidelines and subscribe/unsubscribe info: <a
              href="http://lists.ensembl.org/mailman/listinfo/dev"
              rel="noreferrer" target="_blank" moz-do-not-send="true">http://lists.ensembl.org/<wbr>mailman/listinfo/dev</a><br>
            Ensembl Blog: <a href="http://www.ensembl.info/"
              rel="noreferrer" target="_blank" moz-do-not-send="true">http://www.ensembl.info/</a><br>
            <br>
          </blockquote>
        </div>
        <br>
      </div>
      <br>
      <fieldset class="mimeAttachmentHeader"></fieldset>
      <br>
      <pre wrap="">_______________________________________________
Dev mailing list    <a class="moz-txt-link-abbreviated" href="mailto:Dev@ensembl.org">Dev@ensembl.org</a>
Posting guidelines and subscribe/unsubscribe info: <a class="moz-txt-link-freetext" href="http://lists.ensembl.org/mailman/listinfo/dev">http://lists.ensembl.org/mailman/listinfo/dev</a>
Ensembl Blog: <a class="moz-txt-link-freetext" href="http://www.ensembl.info/">http://www.ensembl.info/</a>
</pre>
    </blockquote>
    <br>
    <div class="moz-signature">-- <br>
      <b><big>Wasiu Ajenifuja Akanni</big></b><br>
      Developer<br>
      <br>
      Compara group<br>
      EMBL-EBI<br>
      Phone: + 44 (0) 1223 494 237<br>
      Room A3145, West building | Wellcome Trust Genome Campus | Hinxton
      | Cambridge | CB10 1SD | UK</div>
  </body>
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