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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;"><font face="Arial" size="2">Hi,</font>
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<div><font face="Arial" size="2">I have been trying to use the dbNSFP database to annotate my variants. Currently, I'm trying to use the latest version of the database (v4.0), but I have also tried using the version 3.5. With both versions, I'm getting the
following error message whenever I'm trying to run it -</font></div>
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<p style="margin-right: 0px; margin-left: 0px; line-height: normal;"><font face="Arial" size="2"><b>Failed to instantiate plugin dbNSFP: ERROR: Could not read headers from dbNSFP4.0b2a.txt.gz</b></font></p>
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<p style="margin-right: 0px; margin-left: 0px; line-height: normal;"><font face="Arial" size="2">When I zcat the database file, I am able to read the header line, which was obtained from one of the chromosome files, as suggested in the online manual.</font></p>
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<p style="margin-right: 0px; margin-left: 0px; line-height: normal;"><font face="Arial" size="2">The formatting steps for the database (including tab indexing) seem to have gone fine, so I'm not sure where the problem is. Also, I am being able to use the other
VEP Plugins just fine; for now, I'm getting this error messge with only the dbNSFP plugin.</font></p>
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<p style="margin-right: 0px; margin-left: 0px; line-height: normal;"><font face="Arial" size="2">It would be great if I could have some pointers regarding where the issue might be. </font></p>
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<div><font face="Arial" size="2">Many Thanks</font></div>
<div><font face="Arial" size="2">Abhishek</font></div>
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